Shaorong (Ron) Chong
Shaorong (Ron) Chong
Arbor Biotechnologies
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Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
S Chong, FB Mersha, DG Comb, ME Scott, D Landry, LM Vence, ...
Gene 192 (2), 271-281, 1997
Protein splicing involving the Saccharomyces cerevisiae VMA intein: the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in …
S Chong, Y Shao, H Paulus, J Benner, FB Perler, MQ Xu
Journal of Biological Chemistry 271 (36), 22159-22168, 1996
Cas13d is a compact RNA-targeting type VI CRISPR effector positively modulated by a WYL-domain-containing accessory protein
WX Yan, S Chong, H Zhang, KS Makarova, EV Koonin, DR Cheng, ...
Molecular cell 70 (2), 327-339. e5, 2018
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
S Chong, GE Montello, A Zhang, EJ Cantor, W Liao, MQ Xu, J Benner
Nucleic acids research 26 (22), 5109-5115, 1998
Characterization of a self-splicing mini-intein and its conversion into autocatalytic N-and C-terminal cleavage elements: facile production of protein building blocks for …
S Mathys, TC Evans Jr, IC Chute, H Wu, S Chong, J Benner, XQ Liu, ...
Gene 231 (1-2), 1-13, 1999
Functionally diverse type V CRISPR-Cas systems
WX Yan, P Hunnewell, LE Alfonse, JM Carte, E Keston-Smith, ...
Science 363 (6422), 88-91, 2019
Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
S Chong, KS Williams, C Wotkowicz, MQ Xu
Journal of Biological Chemistry 273 (17), 10567-10577, 1998
Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs
S Chong, MQ Xu
Journal of Biological Chemistry 272 (25), 15587-15590, 1997
Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised …
DG Comb, FB Perler, WE Jack, MQ Xu, RA Hodges, CJ Noren, ...
US Patent 5,834,247, 1998
[24] Fusions to self-splicing inteins for protein purification
MQ Xu, H Paulus, S Chong
Methods in enzymology 326, 376-418, 2000
Overview of cell‐free protein synthesis: historic landmarks, commercial systems, and expanding applications
S Chong
Current protocols in molecular biology 108 (1), 16.30. 1-16.30. 11, 2014
A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based
JR Hillebrecht, S Chong
BMC biotechnology 8 (1), 1-9, 2008
Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli
SS Sharma, S Chong, SW Harcum
Journal of biotechnology 125 (1), 48-56, 2006
Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex
H Wang, S Chong
Proceedings of the National Academy of Sciences 100 (2), 478-483, 2003
In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
H Asahara, S Chong
Nucleic acids research 38 (13), e141-e141, 2010
Intein-mediated protein ligation of expressed proteins
TC Evans, MQ Xu, S Chong
US Patent 6,849,428, 2005
Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional …
Y Zhou, H Asahara, EA Gaucher, S Chong
Nucleic acids research 40 (16), 7932-7945, 2012
Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
A Zhang, SM Gonzalez, EJ Cantor, S Chong
Gene 275 (2), 241-252, 2001
tRNA-guanine transglycosylase from Escherichia coli: overexpression, purification and quaternary structure
GA Garcia, KA Koch, S Chong
Journal of molecular biology 231 (2), 489-497, 1993
Intein-mediated rapid purification of Cre recombinase
EJ Cantor, S Chong
Protein expression and purification 22 (1), 135-140, 2001
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