Stephen Bustin
Stephen Bustin
Professor of Molecular Medicine, Anglia Ruskin University
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The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
SA Bustin, V Benes, JA Garson, J Hellemans, J Huggett, M Kubista, ...
Clinical chemistry 55 (4), 611-622, 2009
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
SA Bustin
Journal of molecular endocrinology 25 (2), 169-193, 2000
INVITED REVIEW Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
SA Bustin
Journal of molecular endocrinology 29, 23-39, 2002
Quantification of mRNA using real-time RT-PCR
T Nolan, RE Hands, SA Bustin
Nature protocols 1 (3), 1559-1582, 2006
Real-time RT-PCR normalisation; strategies and considerations
J Huggett, K Dheda, S Bustin, A Zumla
Genes & Immunity 6 (4), 279-284, 2005
The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans
S Gnanapavan, B Kola, SA Bustin, DG Morris, P McGee, P Fairclough, ...
The journal of clinical endocrinology & metabolism 87 (6), 2988-2991, 2002
Quantitative real-time RT-PCR–a perspective
SA Bustin, V Benes, T Nolan, MW Pfaffl
Journal of molecular endocrinology 34 (3), 597-601, 2005
Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction
SA Bustin, T Nolan
Journal of biomolecular techniques: JBT 15 (3), 155, 2004
Validation of housekeeping genes for normalizing RNA expression in real-time PCR
K Dheda, JF Huggett, SA Bustin, MA Johnson, G Rook, A Zumla
Biotechniques 37 (1), 112-119, 2004
Parameters for successful PCR primer design
SA Bustin, R Mueller, T Nolan
Quantitative Real-Time PCR: Methods and Protocols, 5-22, 2020
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
JF Huggett, CA Foy, V Benes, K Emslie, JA Garson, R Haynes, ...
Clinical chemistry 59 (6), 892-902, 2013
Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies
C Tricarico, P Pinzani, S Bianchi, M Paglierani, V Distante, M Pazzagli, ...
Analytical biochemistry 309 (2), 293-300, 2002
The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization
K Dheda, JF Huggett, JS Chang, LU Kim, SA Bustin, MA Johnson, ...
Analytical biochemistry 344 (1), 141-143, 2005
MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
SA Bustin, JF Beaulieu, J Huggett, R Jaggi, FSB Kibenge, PA Olsvik, ...
BMC molecular biology 11, 1-5, 2010
Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
SA Bustin, R Mueller
Clinical Science 109 (4), 365-379, 2005
AZ of quantitative PCR
SA Bustin
International University Line, 2004
The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors
M Korbonits, SA Bustin, M Kojima, S Jordan, EF Adams, DG Lowe, ...
The journal of clinical endocrinology & metabolism 86 (2), 881-887, 2001
Why the need for qPCR publication guidelines?—The case for MIQE
SA Bustin
Methods 50 (4), 217-226, 2010
Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians
BW Ogunkolade, BJ Boucher, JM Prahl, SA Bustin, JM Burrin, K Noonan, ...
Diabetes 51 (7), 2294-2300, 2002
SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations
T Nolan, RE Hands, W Ogunkolade, SA Bustin
Analytical biochemistry 351 (2), 308-310, 2006
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